Objectives 5:
Genetic mapping in crop legumes

5a Provide novel germplasm for trait analysis (Fast Neutron mutant analysis and mapping)
5b Integrated Genetic Maps
Objective 5a will be achieved by:


a) selecting a set of 12 independent M3 individuals from a novel legume genetic resource [fast neutron (FN) deletion pea population]

b) performing cDNA-AFLP analysis of gene transcripts from total plants of these individuals and determining the number of genes deleted per FN line

c) sequencing the cDNA-AFLP fragments corresponding to the missing transcripts and identifying the corresponding genes

d) performing marker analysis of the deleted fragments by mapping identified genes in extant mapping RI populations

Objective 5b will involve:


e) identifying the most effective strategy for relating the genetic maps of pea, field bean and lupin for UK use

f) establishing genetic mapping populations for bean and lupin within UK through coordination with the EU Grain Legumes project

g) designing a comprehensive set of gene-based markers (at least 100) to enable integration of gene maps across crop species

h) defining a set of molecular markers for priority traits that can be exploited by breeders