Objectives 4:
Reverse genetics

TILLING for genes that regulate the development of the aerial part of the legume plant.
This will involve:


a) sequencing 5000 random cDNAs (ESTs) from a cDNA library generated from pea shoots

b) annotating sequences and identifying targets for TILLING, based on database mining and identity of orthologous genes involved in plant shoot architecture

c) designing primers based on candidate genes, derived from a) and b) and from databases developed within the EU project (Grain Legumes)

d) TILLING for mutants in these genes, using the platform to be developed within the EU project (Grain Legumes) and assessing phenotypes of mutants.